Measurement

Part:BBa_K4511007:Design

Designed by: Wenhe Ma   Group: iGEM22_HUS_United   (2022-10-12)


Reporter device with J23106 , B0032, and GFP for dtRNA1 strength measurement


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 753


Design Notes

the distance between promoter and dtRNAs, dtRNAs, and RBSs should be carefully adjusted, and maybe even conduct some research on its effect. Therefore, seamless cloning methods such as HiFi assembly are more suited.


Source

We constructed this device using the HiFi assembly method to integrate dtRNA fragment and BBa_J364009 from the iGEM kit.

References